Protein_Domain
mDNMT1(731

Part:BBa_K1362450:Design

Designed by: Constantin Ahlmann-Eltze, Charlotte Bunne, Magdalena Büscher, Jan Gleixner, Max Horn, Anna Huhn, Nils Klughammer, Jakob Kreft, Elisabeth Schäfer, Carolin Schmelas, Silvan Schmitz, Max Waldha   Group: iGEM14_Heidelberg   (2014-10-08)


Murine DNMT1, residues 731-1602


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 1017
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 2323
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

Source

Mus musculus; codon-optimized for E. coli

References

[1] Song, Jikui; Rechkoblit, Olga; Bestor, Timothy H; Patel, Dinshaw J (2011). "Structure of DNMT1-DNA complex reveals a role for autoinhibition in maintenance DNA methylation". Science 331 (6020): 1236–40